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Secondary metabolites with antimicrobial activity produced by thermophilic bacteria from a high-altitude hydrothermal system

dc.contributor.authorPardo-Esté, Coral
dc.contributor.authorCortés, Johanna
dc.contributor.authorCastro-Severyn, Juan
dc.contributor.authorPérez, Vilma
dc.contributor.authorHenríquez-Aedo, Karem
dc.contributor.authorCuadros, Fabian
dc.contributor.authorYañez, Carolina
dc.contributor.authorCuadros-Orellana, Sara
dc.contributor.authorDorador, Cristina
dc.contributor.authorMolina, Veronica
dc.contributor.authorEissler, Yoanna
dc.contributor.authorPaquis, Pablo
dc.contributor.authorJeffrey, Wade H.
dc.contributor.authorPozo, Patricia
dc.contributor.authorPérez, Pablo A.
dc.contributor.authorHengst, Martha B.
dc.date.accessioned2024-11-27T12:35:56Z
dc.date.available2024-11-27T12:35:56Z
dc.date.issued2024
dc.identifier.urihttp://repositorio.ucm.cl/handle/ucm/5777
dc.description.abstractThermophilic microorganisms possess several adaptations to thrive in high temperature, which is reflected as biosynthesis of proteins and thermostable molecules, isolation and culture represent a great methodological challenge, therefore High throughput sequencing enables screening of the whole bacterial genome for functional potential, providing rapid and cost-effective information to guide targeted cultures for the identification and characterization of novel natural products. In this study, we isolated two thermophilic bacterial strains corresponding to Bacillus LB7 and Streptomyces LB8, from the microbial mats in the Atacama Desert. By combining genome mining, targeted cultures and biochemical characterization, we aimed to identify their capacity to synthesize bioactive compounds with antimicrobial properties. Additionally, we determined the capability to produce bioactive compounds under controlled in vitro assays and detected by determining their masses by Thin-Layer Chromatography/Mass Spectrometry (TLC/MS). Overall, both isolates can produce antimicrobial (e.g., Myxalamide C by-product) and antioxidants (e.g. Dihydroxymandelic Acid, Amide biotine and Flavone by-products) compounds. Bacillus LB7 strain possesses a more diverse repertoire with 51.95% of total metabolites unmatched, while Streptomyces LB8 favors mainly antioxidants, but has over 70% of unclassified compounds, highlighting the necessity to study and elucidate the structure of novel compounds. Based on these results, we postulate that the uncultured or rare cultured thermophiles inhabiting high-altitude hydrothermal ecosystems in the Atacama Desert offer a promising opportunity to the study of novel microbial bioactive compounds.es_CL
dc.language.isoenes_CL
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
dc.sourceFrontiers in Microbiology, 15, art. no. 1477458es_CL
dc.subjectBioprospectiones_CL
dc.subjectSecondary metaboliteses_CL
dc.subjectThermophilic bacteriaes_CL
dc.subjectAntimicrobial activityes_CL
dc.subjectBioactive compoundses_CL
dc.titleSecondary metabolites with antimicrobial activity produced by thermophilic bacteria from a high-altitude hydrothermal systemes_CL
dc.typeArticlees_CL
dc.ucm.indexacionScopuses_CL
dc.ucm.indexacionIsies_CL
dc.ucm.urifrontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1477458/full#h1es_CL
dc.ucm.doidoi.org/10.3389/fmicb.2024.1477458es_CL


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Atribución-NoComercial-SinDerivadas 3.0 Chile
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